No, when you sign-in to Serato DJ Pro, it will activate your Serato DJ Pro license for that computer.

This means that you will be able to connect any of the supported Serato DJ Lite hardware unlocked devices to your computer and the software will remain activated, regardless of the specific compatible controller you have connected.

You can deactivate unwanted computers, or activate a new or alternative computer, using our How to deactivate your Serato DJ products & licenses to use on another computer article.