Serato Sample has two playback modes ; trigger and hold modes.
Trigger mode means that triggering a pad will continue playback until the end of the sample track, or until the end of the Cue Point region.
Hold mode means that triggering a pad will continue playback until the pad is released.
Setting a Cue Point end is easy, simply drag the bottom arrow of a cue point to the right. This will now send an endpoint where the playhead will stop once it reaches.
The 16 Serato Sample pads correspond with 16 MIDI notes on the host DAW's MIDI piano roll. This will work just like using any other VST or MIDI instrument. The pads can be triggered via computer keyboard - just make sure the MIDI focus for your keyboard starts at octave C1, if it doesn't just change the octave on your DAW. (Note : In some DAWs, like Fruity Loops, it starts at C3). There are letters on the pads to let you know which key triggers which pad (note : this is only for the officially supported DAW hosts). You can also trigger the pads from a connected MIDI controller - providing the notes start at C1 (most MIDI controllers will have access to these notes).
Note: On both Ableton Live and Logic Pro, you can quickly shift octaves by using 'z' and 'x' keyboard shortcuts to get into the correct range. You can learn how to change the 'Root Key' of the channel in FL Studio, here.
Check out the video below, for more info and tips on playback;